| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111293 |
| Chromosome: | chromosome_1 |
| Location: | 1747302 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009350 | AIH2 | Agmatine iminohydrolase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCCGACCCCTCGGTACGTGTCATGACCCG |
| Internal bar code: | GCGGTTTTAATTTACAAGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 98.2768 |
| LEAP-Seq n confirming: | 5190 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCTCCTCCACCTGACT |
| Suggested primer 2: | GCTCGCGTTATTCTGCTACC |