| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111295 |
| Chromosome: | chromosome 10 |
| Location: | 4477484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGAGGAGAAGGCGGAGTGCGCGCGGAG |
| Internal bar code: | GCGCAGGATGACGCGGGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 89.9975 |
| LEAP-Seq n confirming: | 3554 |
| LEAP-Seq n nonconfirming: | 395 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCAAATCTGTCAGAGCG |
| Suggested primer 2: | CGAGGTAGCCAGCAATAAGC |