| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.111323 |
| Chromosome: | chromosome 3 |
| Location: | 1227618 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149750 | DMA2 | (1 of 2) 2.1.1.297 - Peptide chain release factor N(5)-glutamine methyltransferase / N(5)-glutamine S-adenosyl-L-methionine dependent methyltransferase; Putative DNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCACGGCCCCACTCACAACGACACGCC |
| Internal bar code: | CTCAGTGCATATCATTAACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 98.6207 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAATACTGCTGTGGGACC |
| Suggested primer 2: | AGTGATTCATTCACCCAGGC |