| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.111328 |
| Chromosome: | chromosome 3 |
| Location: | 3774014 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAAAGAGGCTGCTGCACATGCGCATTC |
| Internal bar code: | AGGGCGGTCCAAGGTAGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 98.3193 |
| LEAP-Seq n confirming: | 585 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCGGGATACAGAAGCG |
| Suggested primer 2: | GAGCTACCTGCACAATGCAA |