Insertion junction: LMJ.RY0402.111334_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111334
Chromosome:chromosome 9
Location:3448620
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389319 (1 of 2) PTHR11266:SF17 - PROTEIN MPV17 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATTGATTGACCCCCGTCATGTTGTCTGC
Internal bar code:CTCGTAGCACTGAGTAACAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:909
LEAP-Seq percent confirming:98.983
LEAP-Seq n confirming:7592
LEAP-Seq n nonconfirming:78
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTGACGTCTGCTTCCTG
Suggested primer 2:CTAGATCACGCCAACCCAAT