| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111335 |
| Chromosome: | chromosome 1 |
| Location: | 4868388 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033700 | (1 of 1) PF01933 - Uncharacterised protein family UPF0052 (UPF0052) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGAAGTTCGCACACGACACTTCCTCCAC |
| Internal bar code: | TTTATTCCCCGACCGCGCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 532 |
| LEAP-Seq percent confirming: | 39.8768 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 1366 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGTGGAGACCAACAAGG |
| Suggested primer 2: | ATGCGAACGGTGTGTATCAA |