| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111338 |
| Chromosome: | chromosome 7 |
| Location: | 1063547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319650 | FXL4,FXL5 | FixL-like PAS domain protein; (1 of 3) IPR000014//IPR013767 - PAS domain // PAS fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGTAGGTTTAGTATACCCATGAGCAGG |
| Internal bar code: | TAGCGGAGCGAGGTCGGGCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 61.256 |
| LEAP-Seq n confirming: | 12046 |
| LEAP-Seq n nonconfirming: | 7619 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTCGATTACGGAAGGCA |
| Suggested primer 2: | CGGGCACTTTAACCAGTTGT |