| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111343 |
| Chromosome: | chromosome 2 |
| Location: | 646223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078000 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTAGCGAAGCATTCAATTCGATTTGAT |
| Internal bar code: | TCTTTCTAATCTCGCTCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 95.5674 |
| LEAP-Seq n confirming: | 539 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAATCTTGTGCAACAGC |
| Suggested primer 2: | CCACCTCAATCTGTCCACCT |