| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111424 |
| Chromosome: | chromosome 11 |
| Location: | 1278587 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467706 | (1 of 1) PTHR10625//PTHR10625:SF117 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCGTATGTGTGTCCATGTGTGCGTATG |
| Internal bar code: | GGGTGGCTGCGTCGCCGACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 10.8374 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 1267 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGTTCTGGGGTGACAG |
| Suggested primer 2: | ATGCCGTAGCGTAAATGACC |