| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111485 |
| Chromosome: | chromosome 12 |
| Location: | 7656406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554550 | (1 of 1) IPR026771 - Transmembrane protein 218 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAATGGTCTGCCGCGCCGCAACGCCCAT |
| Internal bar code: | GAGGTCAGTGGTAGGCGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.5442 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGCAAGTTTATGTGCG |
| Suggested primer 2: | GCCGTATGTGAGTATGGCCT |