| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111581 |
| Chromosome: | chromosome 2 |
| Location: | 4178444 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098400 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTTCATAACTCTGTTGCGGGTGCGAGC |
| Internal bar code: | TTGACCCGACTGCACGCAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 98.7711 |
| LEAP-Seq n confirming: | 643 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTTGATAGTCGCCTGCC |
| Suggested primer 2: | CATGCGTGTAAGTACGGTGC |