| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111601 |
| Chromosome: | chromosome 17 |
| Location: | 4483265 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACATGCAAGTACGTGGGCGCCCGCTTC |
| Internal bar code: | CAATGGCTGCAGAAGTCAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.0811 |
| LEAP-Seq n confirming: | 3127 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGGATGATCCGCTATTG |
| Suggested primer 2: | CTCATGTGTGTGTCAAGGGG |