| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.111607 |
| Chromosome: | chromosome 6 |
| Location: | 5108054 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281000 | (1 of 52) PF00856 - SET domain (SET) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCTAGCCCGGTGCAGCCACAGGTGCAG |
| Internal bar code: | AAAAAACGGTATGTGACATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 99.5798 |
| LEAP-Seq n confirming: | 3792 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCTGGTGCGAAGAAAT |
| Suggested primer 2: | ATGCTTGCGTGCATGATAAC |