| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111626 |
| Chromosome: | chromosome 11 |
| Location: | 608540 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467612 | (1 of 1) PTHR10108//PTHR10108:SF924 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAAACAAAGCGCAAAGACAGTGCAGGTG |
| Internal bar code: | ACCGGCATCGCCGGAGAACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.3117 |
| LEAP-Seq n confirming: | 2453 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCTCGTTCTTGAACCC |
| Suggested primer 2: | GCTCGTTTAGCTTGGTTTGG |