| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111627 |
| Chromosome: | chromosome 17 |
| Location: | 705268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700950 | FDX5 | (1 of 2) PTHR19370//PTHR19370:SF110 - NADH-CYTOCHROME B5 REDUCTASE // SUBFAMILY NOT NAMED; Ferredoxin 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATTGAACACCTTGACTCGCGCCTTGAC |
| Internal bar code: | ATAATGATCGCAAGGCGCTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 98.7294 |
| LEAP-Seq n confirming: | 2642 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGCAAGACGAAGACTAT |
| Suggested primer 2: | CGGCATACACACATGACACA |