Insertion junction: LMJ.RY0402.111650_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111650
Chromosome:chromosome 9
Location:2529998
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390957 (1 of 6) IPR001471//IPR016177 - AP2/ERF domain // DNA-binding domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCGAGTAGTGCATGTCGCATCAGCCTGC
Internal bar code:ACCGGGATTTTCGCCTAACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2239
LEAP-Seq percent confirming:34.362
LEAP-Seq n confirming:4721
LEAP-Seq n nonconfirming:9018
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCATCAAGATCATCGCC
Suggested primer 2:TCATTATTCGGTTTCACGCA