| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111651 |
| Chromosome: | chromosome 2 |
| Location: | 4314771 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g099100 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP); RCC-type HECT ubiquitin ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTCCGACTTTCCAGTATTGATTCATGA |
| Internal bar code: | CGGCGTGCCACTGCGGAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1234 |
| LEAP-Seq percent confirming: | 99.2966 |
| LEAP-Seq n confirming: | 1694 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAAGGACCAAAAATGGG |
| Suggested primer 2: | AAATTATATGCACGGCAGGC |