Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.111704 |
Chromosome: | chromosome_9 |
Location: | 1756442 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g396451 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCACCAGATGGGCGAGTACGGCAGATACA |
Internal bar code: | CAGCGCCGTCGTCGGTCATGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 517 |
LEAP-Seq percent confirming: | 99.7558 |
LEAP-Seq n confirming: | 17156 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCAGTAGTTGGCAATCCCC |
Suggested primer 2: | GTAGTGAACATGCCGTCCCT |