| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111707 |
| Chromosome: | chromosome_17 |
| Location: | 3426843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724400 | FAP257 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCGGAGTTCGGGCGCTGCGCATAGCGGCT |
| Internal bar code: | AGAACATACCCTGTAGGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 97.7401 |
| LEAP-Seq n confirming: | 2422 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTAATTCGAGAGCAGGGC |
| Suggested primer 2: | CCGGATTCAGGAGATTACGA |