| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111721 |
| Chromosome: | chromosome 3 |
| Location: | 4295624 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTACGCCGGCGTTCGACCAGCCATGCA |
| Internal bar code: | GTCAGCCACGAAAGACTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 86.9551 |
| LEAP-Seq n confirming: | 2693 |
| LEAP-Seq n nonconfirming: | 404 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAGTGGGATTGCAAGGT |
| Suggested primer 2: | ACTCAAGCAGACGCAGACAA |