Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.111721 |
Chromosome: | chromosome 3 |
Location: | 4295626 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g174650 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCGAGCATGGGGTGGGATTCTGAGCTT |
Internal bar code: | TTACCCTTGCCTATGCCTCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 629 |
LEAP-Seq percent confirming: | 57.0755 |
LEAP-Seq n confirming: | 484 |
LEAP-Seq n nonconfirming: | 364 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCAAGCAGACGCAGACAA |
Suggested primer 2: | GTTGAGTGGGATTGCAAGGT |