Insertion junction: LMJ.RY0402.111721_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111721
Chromosome:chromosome 3
Location:4295626
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174650 gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCGAGCATGGGGTGGGATTCTGAGCTT
Internal bar code:TTACCCTTGCCTATGCCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:629
LEAP-Seq percent confirming:57.0755
LEAP-Seq n confirming:484
LEAP-Seq n nonconfirming:364
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAAGCAGACGCAGACAA
Suggested primer 2:GTTGAGTGGGATTGCAAGGT