Insertion junction: LMJ.RY0402.111740_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.111740
Chromosome:chromosome 2
Location:2510297
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g092250 FKB42,FKB22 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PF00254//PF07719//PF13414 - FKBP-type peptidyl-prolyl cis-trans isomerase (FKBP_C) // Tetratricopeptide repeat (TPR_2) // TPR repeat (TPR_11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTATCCCGATTGCCCCTATGCCAGACATC
Internal bar code:TGCTAGTACGTCGATCGGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:62.0491
LEAP-Seq n confirming:2907
LEAP-Seq n nonconfirming:1778
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CACCGAGCTAAAGAAGTCCG
Suggested primer 2:CGCATTCTACAAGACCAGCA