| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111743 |
| Chromosome: | chromosome 16 |
| Location: | 4638215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687550 | CGL99 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCCGAACGGCCAAGGGAGCGGGCGAA |
| Internal bar code: | TTTGTTCGTATTATTAAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 99.4787 |
| LEAP-Seq n confirming: | 4389 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCACCTGTCTTGCGA |
| Suggested primer 2: | GAAGCTCAAGGTAAGTGCGG |