Insertion junction: LMJ.RY0402.111746_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111746
Chromosome:chromosome 11
Location:2482807
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGATCGCCGCAACTACTTCTTGGTTGCGG
Internal bar code:GAGGGTGCCGAGCGGGAGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:424
LEAP-Seq percent confirming:98.98
LEAP-Seq n confirming:6793
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTTGGCTGCAACTCCTT
Suggested primer 2:GGCGACACAGTGGTAAAGGT