Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.111746 |
Chromosome: | chromosome_11 |
Location: | 2482807 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g475600 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGATCGCCGCAACTACTTCTTGGTTGCGG |
Internal bar code: | GAGGGTGCCGAGCGGGAGTTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 424 |
LEAP-Seq percent confirming: | 98.98 |
LEAP-Seq n confirming: | 6793 |
LEAP-Seq n nonconfirming: | 70 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATTTTGGCTGCAACTCCTT |
Suggested primer 2: | GGCGACACAGTGGTAAAGGT |