| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111757 |
| Chromosome: | chromosome 17 |
| Location: | 3401810 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724100 | 5'UTR|5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGGCTCACCACGACCTTGGCGGGGCA |
| Internal bar code: | TCTGTCGCAGGGGCCATTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 98.9943 |
| LEAP-Seq n confirming: | 689 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAATAATGCGTGGGTCC |
| Suggested primer 2: | GCTGTCGTGGCGTAACTGTA |