| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111761 |
| Chromosome: | chromosome 16 |
| Location: | 805267 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647534 | NRX4 | Nucleoredoxin 4; (1 of 3) K17609 - nucleoredoxin [EC:1.8.1.8] (NXN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGAGCTGTGAACGCCCTCCCGGTACC |
| Internal bar code: | AGGGGGTCAACCAGGAGAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 98.8399 |
| LEAP-Seq n confirming: | 1278 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGACTGCAGAGATGGTG |
| Suggested primer 2: | ATAGCGAGGCTGACTCCTGA |