| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111766 |
| Chromosome: | chromosome_16 |
| Location: | 512861 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g692902 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCGGCATCCGGCCCTACCTGCCGGCCGGG |
| Internal bar code: | AAGAGAGCATTGTCGAGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 58.9491 |
| LEAP-Seq n confirming: | 359 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCGAACTACCCTTCCC |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |