| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.111795 |
| Chromosome: | scaffold 18 |
| Location: | 152519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749197 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGCTGTCAATCAATCCGCAAGCCGCG |
| Internal bar code: | CTTTTAGCCGGCCTATTGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.6707 |
| LEAP-Seq n confirming: | 7869 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTACAATCAGCTGGC |
| Suggested primer 2: | AACGTACGGTGTGAACACGA |