| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111826 |
| Chromosome: | chromosome 7 |
| Location: | 6083345 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355400 | CDKH1 | Cyclin dependent kinase; (1 of 1) K08818 - cell division cycle 2-like [EC:2.7.11.22] (CDC2L) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTCCGTTCAGGAGTTTACATACCAGG |
| Internal bar code: | GTGGGCGCTGTGGCCGCCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 99.7519 |
| LEAP-Seq n confirming: | 402 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATTCCTTCCCTTCCTC |
| Suggested primer 2: | TGCGCGCTAAACACTTATTG |