| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111831 |
| Chromosome: | chromosome 7 |
| Location: | 1036583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319550 | (1 of 1) PF10442 - FIST C domain (FIST_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACACGAAGCGCGCACGTGCGCGCGCGT |
| Internal bar code: | GGAATGTTGGCCAAGCCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 95.0068 |
| LEAP-Seq n confirming: | 6983 |
| LEAP-Seq n nonconfirming: | 367 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCTCCTCTCCCACTTCA |
| Suggested primer 2: | AAAAACACAAAGGCGGTGTC |