Insertion junction: LMJ.RY0402.111906_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.111906
Chromosome:chromosome 14
Location:1869011
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTTAGTTTGGCGTGCGATGCAATCATT
Internal bar code:TGTGGGACCCACGTGTCCCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:99.4512
LEAP-Seq n confirming:3262
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCATCAGCCACTTGTGAA
Suggested primer 2:GCCTTGGAGCAGCAGTAATC