Insertion junction: LMJ.RY0402.111924_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.111924
Chromosome:chromosome 11
Location:282215
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467560 DYX1C1,PF23,DNAAF4 Dynein assembly factor PF23; (1 of 1) PTHR22904//PTHR22904:SF10 - TPR REPEAT CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCAGCGCACCCACACCCTCACCCTGATT
Internal bar code:GCTAGTCTCTCAATCGTGATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:99.6652
LEAP-Seq n confirming:5060
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTAATTTGGGCTGGTA
Suggested primer 2:TGCTGCGTCATCTGAAGAAC