| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111950 |
| Chromosome: | chromosome 12 |
| Location: | 9293326 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541150 | (1 of 3) PF03942 - DTW domain (DTW) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGGTGCACGCGCACCACGGCGAGTGG |
| Internal bar code: | GTTATTACAACTTGCCATATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 99.2337 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCGTACAGCAGCATCTC |
| Suggested primer 2: | GCGCACAGACTACATCCTCA |