| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111958 |
| Chromosome: | chromosome 6 |
| Location: | 2208363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266100 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCTCAGGGGTGAGCTGCCCGCCAGAC |
| Internal bar code: | AATTTGGACCACCATTCTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.7341 |
| LEAP-Seq n confirming: | 7877 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTTTCTCAAATGCGTC |
| Suggested primer 2: | TTTTGTCCACGAGACTGACG |