| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111964 |
| Chromosome: | chromosome 10 |
| Location: | 5281171 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457400 | (1 of 28) PTHR31600//PTHR31600:SF2 - FAMILY NOT NAMED // TINY MACROCYSTS PROTEIN B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCGGTGAGCACGTGGTACAAGCTATACT |
| Internal bar code: | AGACCCTATGTACTGTTTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.6389 |
| LEAP-Seq n confirming: | 6070 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGACACGCTGGCAACTT |
| Suggested primer 2: | ACCTCGGGTAAGACGCCTAT |