| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112043 |
| Chromosome: | chromosome 10 |
| Location: | 3325690 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443650 | (1 of 1) K15627 - tether containing UBX domain for GLUT4 (ASPSCR1, ASPL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGAGAGAGGAGGTAGAACGGTGCGGG |
| Internal bar code: | CTAACTCTTTACTGATTTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.6333 |
| LEAP-Seq n confirming: | 815 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGTCACACATGGAACC |
| Suggested primer 2: | GAATAGCGGAAGCACAAAGC |