| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112060 |
| Chromosome: | chromosome 5 |
| Location: | 3227137 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCTACCGCCGCAGCCCACCTCTGACAG |
| Internal bar code: | GGGCAGATCAGGGCGCATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 55.0868 |
| LEAP-Seq n confirming: | 666 |
| LEAP-Seq n nonconfirming: | 543 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGTCTGTGTGCTGTGT |
| Suggested primer 2: | CTACCTCAACCTCATCCCGA |