| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112079 |
| Chromosome: | chromosome 14 |
| Location: | 2730173 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626800 | OPR64 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTCCACGCCAACAGCTGCTCCACCCTCC |
| Internal bar code: | GGTTATCTTTATTGGTCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 14.8008 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 449 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGACCTGGAACGAC |
| Suggested primer 2: | GGAGGACGACTCTGAAGACG |