| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112086 |
| Chromosome: | chromosome 16 |
| Location: | 4795366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686400 | FAO13 | FAD-dependent oxidoreductase; (1 of 1) PTHR10742//PTHR10742:SF307 - AMINE OXIDASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGTGGAAGTGTGGGCGGGCTTGTGG |
| Internal bar code: | GGGCCCCTCTGCCGTTTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.0006 |
| LEAP-Seq n confirming: | 6142 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACCTGAAGAGGCAGAG |
| Suggested primer 2: | CACACACACACACACACCGT |