| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112086 |
| Chromosome: | chromosome 16 |
| Location: | 4795368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686400 | FAO13 | FAD-dependent oxidoreductase; (1 of 1) PTHR10742//PTHR10742:SF307 - AMINE OXIDASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATGCCCACTCTCCCTCACACCCTCCC |
| Internal bar code: | GTGGCGTCAAGGATTCGCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 99.1305 |
| LEAP-Seq n confirming: | 61568 |
| LEAP-Seq n nonconfirming: | 540 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCACCTGAAGAGGCAGAG |
| Suggested primer 2: | CACACACACACACACACCGT |