| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112114 |
| Chromosome: | chromosome 5 |
| Location: | 1803053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242178 | (1 of 1) PTHR23344//PTHR23344:SF10 - GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTCGGCGCCACGGGCGCATGCCGGAGC |
| Internal bar code: | TGGTTATAGAGTCGGCGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.122 |
| LEAP-Seq n confirming: | 1129 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATTGGTTCCTAAGCCC |
| Suggested primer 2: | CCCTACCCCAACACAAACAC |