Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.112132 |
Chromosome: | chromosome_16 |
Location: | 7587398 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g689423 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGCATTGGGCGCCGCCAGGGAGCAAACT |
Internal bar code: | GCGGCACAGGCGAGCCCCACCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 171 |
LEAP-Seq percent confirming: | 99.8168 |
LEAP-Seq n confirming: | 545 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGTAGGCCATTGTCCAGC |
Suggested primer 2: | TGAACTAACACCGACCCACA |