| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112132 |
| Chromosome: | chromosome 16 |
| Location: | 7587455 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689423 | (1 of 14) PF00892 - EamA-like transporter family (EamA); Putative UDP-galactose transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGACCCGGCGTGCAGGCATGCGGGTTT |
| Internal bar code: | CGGACATAACCGCCGGTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 98 |
| LEAP-Seq percent confirming: | 99.8817 |
| LEAP-Seq n confirming: | 2532 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTAGGCCATTGTCCAGC |
| Suggested primer 2: | TGAACTAACACCGACCCACA |