| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.112175 |
| Chromosome: | chromosome 16 |
| Location: | 231476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694550 | (1 of 726) IPR011009 - Protein kinase-like domain; Serine/threonine protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGATAGATAAAGAAATAGAGCGCGTAAA |
| Internal bar code: | CGGGTCGTTTTCCTTGTTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1239 |
| LEAP-Seq percent confirming: | 99.6453 |
| LEAP-Seq n confirming: | 3090 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCTTGGGAGTTTCCCTG |
| Suggested primer 2: | CTGCACATAATGCGCTGACT |