| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112223 |
| Chromosome: | chromosome 6 |
| Location: | 3587380 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278121 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGATGACATGCCCGCGCCTCAGGAGG |
| Internal bar code: | TCTTGCTGTCTGTCGAGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.7717 |
| LEAP-Seq n confirming: | 874 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGACGCATTCCTTTTA |
| Suggested primer 2: | GGCGGCTGTACGGTATTTTA |