| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.112224 |
| Chromosome: | chromosome 7 |
| Location: | 1712628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325710 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCGGCCGACCCCGCTTGGCCGTGACG |
| Internal bar code: | ACGGATTTCTAAATCGTTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 77.6339 |
| LEAP-Seq n confirming: | 1319 |
| LEAP-Seq n nonconfirming: | 380 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGTGAGTATGCAAGGT |
| Suggested primer 2: | CAGCTTACCTCCTGCCAAAG |