Insertion junction: LMJ.RY0402.112242_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.112242
Chromosome:chromosome 1
Location:7896825
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g063632 (1 of 1) K03660 - N-glycosylase/DNA lyase (OGG1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCAGCACCAATCAGGGGAATGCGCTTCC
Internal bar code:GCATCTAGCCAAAGAATATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:779
LEAP-Seq percent confirming:99.8467
LEAP-Seq n confirming:3909
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CACAAGGATGTGGTTCAACG
Suggested primer 2:CCAGTGGAACATGGTGTACG