| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.112367 |
| Chromosome: | chromosome 12 |
| Location: | 5086162 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526700 | RBL9 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGAAAGATTGATGGAGCCCGACAGAGT |
| Internal bar code: | TTGGAAAAATGGAGCGGCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.4676 |
| LEAP-Seq n confirming: | 1121 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTCTGTATGCCTGTTCG |
| Suggested primer 2: | CATACATGCAAACACTCCCG |