Insertion junction: LMJ.RY0402.112369_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.112369
Chromosome:chromosome 1
Location:6177180
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g044200 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAGATGTATGCTTTCCCCGCGTTATGCAG
Internal bar code:ATAGAAGCGAGAAATAATGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:877
LEAP-Seq percent confirming:97.5751
LEAP-Seq n confirming:845
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTGTGCTCTTGCTGACC
Suggested primer 2:CCGCTTCCTACCTTGGTGTA