| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.112373 |
| Chromosome: | chromosome 13 |
| Location: | 3425264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587350 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGCACAAGTAATCTATGACATTGTGCG |
| Internal bar code: | GCTCGATTCTGCGGGGGGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.1767 |
| LEAP-Seq n confirming: | 3855 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACGACGTGCTACCCAAT |
| Suggested primer 2: | GTGTGTTTTGATCAGCCCCT |